7/16
If you want perfect mini preps, you have to do the Aynur way. Although I only nano dropped two samples, I got a 490 something for the first one and 350 something for the second one. You just have to wait four minutes after adding P2 buffer, wash with PB twice, spin an extra time after getting rid of PE the first time, and most importantly USE A CENTRIFUGE, NO VACCUMS SORRY! This way takes longer though, but if you want the most plasmid possible its the way to go.
